In-vitro study of bactericidal effect of low-level laser therapy in the presence of photosensitizer on cariogenic bacteria

Iriana Carla J. Zanin; Aldo Brugnera Jr.; Reginaldo Bruno Goncalves

doi:10.1117/12.469318

3 June 2002 In vitro study of bactericidal effect of low-level laser therapy in the presence of photosensitizer on cariogenic bacteria

Iriana Carla J. Zanin, Aldo Brugnera Jr., Reginaldo Bruno Goncalves

Author Affiliations +

Proceedings Volume 4610, Lasers in Dentistry VIII; (2002) https://doi.org/10.1117/12.469318
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The aim of this in vitro study was to determine whether low-level laser light in the presence of a photosensitizer could kill Streptococcus mutans and Streptococcus sobrinus. Suspensions of these microorganisms were exposed to a gallium-aluminium-arsenide laser light (660 nm) in the presence of photosensitizer toluidine blue O. Viable microorganisms were counted on brain heart agar plates after incubation at 37 degree(s)C in partial atmosphere of 10% CO₂ for 48 hours. Their exposure to the laser light in the absence of the dye or the dye in the absence of the laser light presented no significant effect on the viability of the microorganisms. However, a decrease in the number of viable microorganisms was only verified when they were exposed to both the laser light and the dye at the same time. Their total growth inhibition was achieved with a dye concentration of 100 mg/mL and a light energy density of 28.8 J/cm², after being exposed to laser light for 900 seconds. In conclusion, these results imply that these bacteria can be killed by low-power laser light in the presence of the photosensitizer.

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Iriana Carla J. Zanin, Aldo Brugnera Jr., and Reginaldo Bruno Goncalves "In vitro study of bactericidal effect of low-level laser therapy in the presence of photosensitizer on cariogenic bacteria", Proc. SPIE 4610, Lasers in Dentistry VIII, (3 June 2002); https://doi.org/10.1117/12.469318

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