Paper
17 June 2002 Multiwavelength TCSPC lifetime imaging
Wolfgang Becker, Axel Bergmann, Christoph Biskup, Thomas Zimmer, Nikolaj Kloecker, Klaus Benndorf
Author Affiliations +
Abstract
We present a novel time-correlated single photon counting (TCPSC) imaging technique that allows time-resolved multi-wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four-dimensional histogramming process that records the photon density over time, the x-y coordinates of the scanning area and the detector channel number. The histogramming process avoids any time gating or wavelength scanning and therefore yields a near-perfect counting efficiency. Applied to resonance energy transfer (RET) measurements, the setup is capable to record time-resolved fluorescence decays for the donor and the acceptor simultaneously.
© (2002) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Wolfgang Becker, Axel Bergmann, Christoph Biskup, Thomas Zimmer, Nikolaj Kloecker, and Klaus Benndorf "Multiwavelength TCSPC lifetime imaging", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470679
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CITATIONS
Cited by 59 scholarly publications and 2 patents.
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KEYWORDS
Luminescence

Microscopes

Sensors

Resolution enhancement technologies

Laser scanners

Clocks

Photodetectors

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