In vivo imaging of small animals with optical tomography and near-infrared fluorescent probes

Matthew R. Palmer; Yasushi Shibata; Jonathan B. Kruskal M.D.; Robert E. Lenkinski

doi:10.1117/12.472078

21 June 2002 In vivo imaging of small animals with optical tomography and near-infrared fluorescent probes

Matthew R. Palmer, Yasushi Shibata, Jonathan B. Kruskal M.D., Robert E. Lenkinski

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472078
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

A developmental optical tomography has been designed for imaging small animals in vivo using near IR fluorophores. The system employs epi-illumination via a 450 W Xe arc lamp, filtered and collimated to illuminate a 10 cm square movable stage. Emission light is filtered then collected by a high- resolution, high quantum efficiency, cooled CCD camera. Stage movement and image acquisition are under the control of a personal computer running system integration and automation software. During an experiment, the anesthetized animal is secured to the stage and up to 200 projections can be acquired over 180 degrees rotation. Angular sampling of the light distribution at a point on the surface is used to determine relative contributions form ballistic and diffuse photons. We have employed the system to investigate a number of applications of in-vivo fluorescent imaging. In dynamic studies, hepatic function has been visualized in nude mice following intravenous injection of indocyanine green (ICG) and cerebrospinal fluid flow as been measured by injection of ICG-lipoprotein conjugate in the subarachnoid space of the lumbar spine followed by dynamic imaging of the brain. Further applications in physiological imaging, cancer detection, and molecular imaging are under investigation in our laboratory.

Citation Download Citation

Matthew R. Palmer, Yasushi Shibata, Jonathan B. Kruskal M.D., and Robert E. Lenkinski "In vivo imaging of small animals with optical tomography and near-infrared fluorescent probes", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472078

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