Overview on some applications of multispectral imaging autofluorescence microscopy in biology and medicine

Giovanni Agati; Franco Fusi; Monica Monici; Riccardo Pratesi

doi:10.1117/12.527511

19 November 2003 Overview on some applications of multispectral imaging autofluorescence microscopy in biology and medicine

Giovanni Agati, Franco Fusi, Monica Monici, Riccardo Pratesi

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Proceedings Volume 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life; (2003) https://doi.org/10.1117/12.527511
Event: 19th Congress of the International Commission for Optics: Optics for the Quality of Life, 2002, Florence, Italy

Abstract

Many cell and tissue components exposed to light of appropriate wavelength exhibit UV-visible emission. This is a spontaneous fluorescence, defined autofluorescence in contrast with that produced by highly fluorescent exogenous probes. This communication deals with the Multispectral Imaging Autofluorescence Microscopy (MIAM) technique, that allows living cell and tissue analysis without use of any reagent or treatment modifying the native conditions of the samples. The technique is suitable for a wide range of applications, practically in all the fields where samples containing fluorescent substances occur. Examples of application in biology and medicine are given, showing the power of the technique in both basic research and diagnostics.

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Giovanni Agati, Franco Fusi, Monica Monici, and Riccardo Pratesi "Overview on some applications of multispectral imaging autofluorescence microscopy in biology and medicine", Proc. SPIE 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life, (19 November 2003); https://doi.org/10.1117/12.527511

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KEYWORDS

Tissues

Diagnostics

Microscopy

Biology

Luminescence

Medicine

Multispectral imaging

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