Flow cytofluorometric monitoring of leukocyte apoptosis in experimental cholera

Ekaterina Y. Lotsmanova; Alexander L. Kravtsov; Ludmila F. Livanova; Irina M. Kobkova; Oleg S. Kuznetsov; Tatyana N. Shchukovskaya; Nina I. Smirnova; Vladimir V. Kutyrev

doi:10.1117/12.518875

13 October 2003 Flow cytofluorometric monitoring of leukocyte apoptosis in experimental cholera

Ekaterina Y. Lotsmanova, Alexander L. Kravtsov, Ludmila F. Livanova, Irina M. Kobkova, Oleg S. Kuznetsov, Tatyana N. Shchukovskaya, Nina I. Smirnova, Vladimir V. Kutyrev

Author Affiliations +

Proceedings Volume 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV; (2003) https://doi.org/10.1117/12.518875
Event: Saratov Fall Meeting 2002 Laser Physics and Photonics, Spectroscopy, and Molecular Modeling III; Coherent Optics of Ordered and Random Media III, 2002, Saratov, Russian Federation

Abstract

Flow cytofluorometric DNA analysis was applied to determine of the relative contents of proliferative (more then 2C DNA per cell) and apoptotic (less then 2C DNA per cell) leukocytes in blood of adult rabbits, challenged with 10,000 times the 50 % effective dose of Vibrio cholerae virulent strain by the RITARD technique. It has been shown that irreversible increase the percentage of cells carrying DNA in the degradation stage brings to disbalance between the genetically controlled cell proliferation and apoptosis that leads to animal death from the cholera infection. Such fatal changes were not observed in challenging of immunized animals that were not died. Thus received data show that the flow cytofluorometric measurements may be used for detection of transgressions in homeostasis during acute infection diseases, for outlet prognosis of the cholera infection.

Citation Download Citation

Ekaterina Y. Lotsmanova, Alexander L. Kravtsov, Ludmila F. Livanova, Irina M. Kobkova, Oleg S. Kuznetsov, Tatyana N. Shchukovskaya, Nina I. Smirnova, and Vladimir V. Kutyrev "Flow cytofluorometric monitoring of leukocyte apoptosis in experimental cholera", Proc. SPIE 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV, (13 October 2003); https://doi.org/10.1117/12.518875

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available