Photo-induced flip-flop of membrane markers monitored by SHG microscopy

Thomas Pons; Jerome Mertz

doi:10.1117/12.500076

9 October 2003 Photo-induced flip-flop of membrane markers monitored by SHG microscopy

Thomas Pons, Jerome Mertz

Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.500076
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Photoisomerization properties of amphiphilic stilbazolium markers are used to provoke photo-induced flip-flop in model lipid bilayer membranes. The flip-flop mechanism and dynamics are determined using simultaneous two-photon excited fluorescence and second harmonic generation microscopy. In absence of illumination, trans- is the dominant conformation, however when an illumination pulse is applied to the membrane markers, photo-induced isomerization provokes a significant increase in the cis- population, whose flip-flop rate was determined to be at least a thousand times greater than that for the trans- marker. Following the illumination pulse, all markers rapidly relax to the trans-conformation.

Citation Download Citation

Thomas Pons and Jerome Mertz "Photo-induced flip-flop of membrane markers monitored by SHG microscopy", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.500076

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available