Shimmering holograms and cellular motion in osteogenic tumors

David D Nolte; Ping Yu; Michael R. Melloch; John J. Turek

doi:10.1117/12.500879

2 October 2003 Shimmering holograms and cellular motion in osteogenic tumors

David D Nolte, Ping Yu, Michael R. Melloch, John J. Turek

Proceedings Volume 5140, Optical Coherence Tomography and Coherence Techniques; (2003) https://doi.org/10.1117/12.500879
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Time-dependent speckle holograms from inside tumor spheroids using short-coherence near-infrared light provide quantitative measures of the state of health of the tumor tissue. Holographic optical coherence imaging (OCI) records full-frame en face images from successive depths inside a tumor in a so-called “flythrough”. When the flythrough is stopped at a specified depth, the holographic features can be classified as variable (relating to cell motility and Brownian motion) or persistent (arising from specific structure such as necrosis inside the tumor) depending on their temporal variations. A strong trend is observed in the ratio of variable-to-persistent features in tumors that are healthy, metabolically poisoned, or chemically cross-linked. Autocorrelation times also reflect this trend. Depth-gated speckle holography provides a means to sample biologically significant areas without the need for cellular-scale spatial resolution, with possible relevance for intraoperative applications.

Citation Download Citation

David D Nolte, Ping Yu, Michael R. Melloch, and John J. Turek "Shimmering holograms and cellular motion in osteogenic tumors", Proc. SPIE 5140, Optical Coherence Tomography and Coherence Techniques, (2 October 2003); https://doi.org/10.1117/12.500879

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KEYWORDS

Tumors

Holography

Holograms

Tissues

Tissue optics

Speckle

Coherence imaging

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