Applicability of photoacoustic measurement for biomechanical characterization: from in vitro engineered tissue characterization to in vivo diagnosis

Miya Ishihara; Masato Sato; Shunichi Sato; Toshiyuki Kikuchi; Masayuki Ishihara; Joji Mochida; Kyosuke Fujikawa; Makoto Kikuchi

doi:10.1117/12.530527

1 July 2004 Applicability of photoacoustic measurement for biomechanical characterization: from in vitro engineered tissue characterization to in vivo diagnosis

Miya Ishihara, Masato Sato, Shunichi Sato, Toshiyuki Kikuchi, Masayuki Ishihara, Joji Mochida, Kyosuke Fujikawa, Makoto Kikuchi

Author Affiliations +

Proceedings Volume 5319, Laser Interaction with Tissue and Cells XV; (2004) https://doi.org/10.1117/12.530527
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We demonstrated a capability of biomechanical characterization by photoacoustic measurement for the purpose of non-invasive functional evaluation of articular cartilage. In this study, the scheme of photoacoustic measurement was improved. For in vivo application, the measurement scheme was changed from a transmittance mode to a reflectance mode in which an optical fiber was coaxially arranged with a piezoelectric transducer. In order to verify the applicability of this measurement for diagnosis of cartilage degeneration, photoacoustic measurements in a reflectance mode were performed using various degenerated cartilages. As a model of degenerated cartilage, cartilage-bone plugs were punched out from a porcine knee joint and treated with trypsin (1 mg/ml). Stress waves were induced by 250-355 nm, 7-ns light pulses delivered through an optical silica fiber from an OPO and were detected by a piezoelectric transducer. The change in relaxation time, which was correlated with the viscosity-elasticity ratio, had a positive correlation with time of trypsin treatment. Our results revealed the applicability of photoacoustic measurement to in vivo preoperative diagnosis of cartilage degeneration.

Citation Download Citation

Miya Ishihara, Masato Sato, Shunichi Sato, Toshiyuki Kikuchi, Masayuki Ishihara, Joji Mochida, Kyosuke Fujikawa, and Makoto Kikuchi "Applicability of photoacoustic measurement for biomechanical characterization: from in vitro engineered tissue characterization to in vivo diagnosis", Proc. SPIE 5319, Laser Interaction with Tissue and Cells XV, (1 July 2004); https://doi.org/10.1117/12.530527

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available