In vivo multiphoton microscopy of deep tissue with gradient index lenses

Michael J. Levene; Daniel A. Dombeck; Rebecca M. Williams; Jesse Skoch; Gregory A. Hickey; Karl A. Kasischke; Raymond P. Molloy; Martin Ingelsson; Edward A. Stern; Jochen Klucken; Brian J. Bacskai; Warren R. Zipfel; Bradley T. Hyman; Watt W. Webb

doi:10.1117/12.537285

21 June 2004 In vivo multiphoton microscopy of deep tissue with gradient index lenses

Michael J. Levene, Daniel A. Dombeck, Rebecca M. Williams, Jesse Skoch, Gregory A. Hickey, Karl A. Kasischke, Raymond P. Molloy, Martin Ingelsson, Edward A. Stern, Jochen Klucken, Brian J. Bacskai, Warren R. Zipfel, Bradley T. Hyman, Watt W. Webb

Author Affiliations +

Proceedings Volume 5323, Multiphoton Microscopy in the Biomedical Sciences IV; (2004) https://doi.org/10.1117/12.537285
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Gradient index lenses enable multiphoton microscopy of deep tissues in the intact animal. In order to assess their applicability to clinical research, we present in vivo multiphoton microscopy with gradient index lenses in brain regions associated with Alzheimer's disease and Parkinson's disease in both transgenic and wild-type mice. We also demonstrate microscopy of ovary in wild type mouse using only intrinsic fluorescence and second harmonic generation, signal sources which may prove useful for both the study and diagnosis of cancer.

Citation Download Citation

Michael J. Levene, Daniel A. Dombeck, Rebecca M. Williams, Jesse Skoch, Gregory A. Hickey, Karl A. Kasischke, Raymond P. Molloy, Martin Ingelsson, Edward A. Stern, Jochen Klucken, Brian J. Bacskai, Warren R. Zipfel, Bradley T. Hyman, and Watt W. Webb "In vivo multiphoton microscopy of deep tissue with gradient index lenses", Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); https://doi.org/10.1117/12.537285

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