Phase imaging digital holography for biological microscopy

Myung K. Kim; Daniel Parshall

doi:10.1117/12.524862

13 July 2004 Phase imaging digital holography for biological microscopy

Myung K. Kim, Daniel Parshall

Proceedings Volume 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI; (2004) https://doi.org/10.1117/12.524862
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

The digital holographic microscopy is being developed, to offer a number of significant advantages and capabilities in phase imaging. The direct availability of both the amplitude and phase information offers a range of versatile processing techniques that can be applied to the complex field data, including the phase imaging which is particularly straightforward in digital holography. One of the techniques we are developing addresses the problem of 2-pi phase discontinuity in the phase image, where most of the conventional phase unwrapping algorithms require subjective intervention for multi-lambda discontinuity. In the present system, we generate two phase maps by digital holography using two different wavelengths. Numerical superposition of the two phase maps results in a new phase map whose effective wavelength is inversely proportional to the difference of the two wavelengths. The axial range can in principle be arbitrarily large compared to the wavelength, while maintaining axial resolution to a fraction of wavelength. We apply the phase imaging digital holography to a number of systems, including the imaging of thin films and biological cells. Preliminary results from these experiments are presented and future development and applications are discussed.

Citation Download Citation

Myung K. Kim and Daniel Parshall "Phase imaging digital holography for biological microscopy", Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); https://doi.org/10.1117/12.524862

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available