The nonlinear imaging theory of second harmonic and third harmonic generations in confocal microscopy

Zhi-lie Tang; Da Xing; Song-hao Liu

doi:10.1117/12.572025

29 July 2004 The nonlinear imaging theory of second harmonic and third harmonic generations in confocal microscopy

Zhi-lie Tang, Da Xing, Song-hao Liu

Proceedings Volume 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics; (2004) https://doi.org/10.1117/12.572025
Event: ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, 2003, Silsoe, United Kingdom

Abstract

The nonlinear imaging theory of second harmonic generation (SHG) and third harmonic generation (THG) in confocal microscopy is presented in this paper. The nonlinear effect of SHG and THG on the imaging properties of confocal microscopy has been analyzed in detail by these imaging theory. It is proved that the imaging process of SHG and THG in confocal microscopy, which is different from conventional coherent imaging or incoherent imaging, can be divided into two different processes of coherent imaging. The three-dimensional point spread functions (3D-PSF) of SHG and THG confocal microscopy are derived based on the nonlinear principles of SHG and THG. The imaging properties of SHG and THG confocal microscopy are discussed in detail according to its 3D-PSF. It is showed that the resolution of SHG and THG confocal microscopy are higher than that of single- and two-photon confocal microscopy.

Citation Download Citation

Zhi-lie Tang, Da Xing, and Song-hao Liu "The nonlinear imaging theory of second harmonic and third harmonic generations in confocal microscopy", Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); https://doi.org/10.1117/12.572025

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