Full-field OCT: applications in ophthalmology

Katharine Grieve; Arnaud Dubois; Michel Paques; Jean-François Le Gargasson; Albert Claude Boccara

doi:10.1117/12.588649

18 April 2005 Full-field OCT: applications in ophthalmology

Katharine Grieve, Arnaud Dubois, Michel Paques, Jean-François Le Gargasson, Albert Claude Boccara

Proceedings Volume 5688, Ophthalmic Technologies XV; (2005) https://doi.org/10.1117/12.588649
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We present images of ocular tissues obtained using ultrahigh resolution full-field OCT. The experimental setup is based on the Linnik interferometer, illuminated by a tungsten halogen lamp. En face tomographic images are obtained in real-time without scanning by computing the difference of two phase-opposed interferometric images recorded by a high-resolution CCD camera. A spatial resolution of 0.7 μm × 0.9 μm (axial × transverse) is achieved thanks to the short source coherence length and the use of high numerical aperture microscope objectives. A detection sensitivity of 90 dB is obtained by means of image averaging and pixel binning. Whole unfixed eyes and unstained tissue samples (cornea, lens, retina, choroid and sclera) of ex vivo rat, mouse, rabbit and porcine ocular tissues were examined. The unprecedented resolution of our instrument allows cellular-level resolution in the cornea and retina, and visualization of individual fibers in the lens. Transcorneal lens imaging was possible in all animals, and in albino animals, transscleral retinal imaging was achieved. We also introduce our rapid acquisition full-field optical coherence tomography system designed to accommodate in vivo ophthalmologic imaging. The variations on the original system technology include the introduction of a xenon arc lamp as source, and rapid image acquisition performed by a high-speed CMOS camera, reducing acquisition time to 5 ms per frame.

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Katharine Grieve, Arnaud Dubois, Michel Paques, Jean-François Le Gargasson, and Albert Claude Boccara "Full-field OCT: applications in ophthalmology", Proc. SPIE 5688, Ophthalmic Technologies XV, (18 April 2005); https://doi.org/10.1117/12.588649

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KEYWORDS

Optical coherence tomography

Imaging systems

Tissues

Image segmentation

Cornea

Image resolution

In vivo imaging

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