In vitro gene transfer by the use of laser-induced stress wave: effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa; Shunichi Sato; Makoto Ogura; Yoshinori Masaki; Maki Uenoyama; Hitoshi Wakisaka; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.588852

15 April 2005 In vitro gene transfer by the use of laser-induced stress wave: effect of laser irradiation conditions on transfection efficiency

Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, Minoru Obara

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Proceedings Volume 5695, Optical Interactions with Tissue and Cells XVI; (2005) https://doi.org/10.1117/12.588852
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Laser-mediated gene transfection is very attractive as a new method for targeted gene therapy because of its high spatial controllability of laser energy. Previously, we demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISW). In this study, we investigated the experimental conditions to increase transfection efficiency in vitro. By applying single-pulse LISW, transfection efficiency was increased with increasing laser fluence. Increase in the number of laser pulses increased transfection efficiency for laser fluences up to 1.3 J/cm², but at higher fluences (>1.7 J/cm²), efficiency showed saturation tendency. Temperature dependence of transfection efficiency was also investigated.

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Mitsuhiro Terakawa, Shunichi Sato, Makoto Ogura, Yoshinori Masaki, Maki Uenoyama, Hitoshi Wakisaka, Hiroshi Ashida, and Minoru Obara "In vitro gene transfer by the use of laser-induced stress wave: effect of laser irradiation conditions on transfection efficiency", Proc. SPIE 5695, Optical Interactions with Tissue and Cells XVI, (15 April 2005); https://doi.org/10.1117/12.588852

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KEYWORDS

In vitro testing

Laser therapeutics

Laser irradiation

Plasma

Laser energy

Laser tissue interaction

In vivo imaging

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