Long-term in vivo harmonics imaging of zebrafish embryonic development based on a femtosecond Cr:forsterite laser

S.-Y. Chen; T.-H. Tsai; C.-S. Hsieh; S.-P. Tai; C.-Y. Lin; C.-Y. Ko; Y.-C. Chen; H.-J. Tsai; C.-H. Hu; C.-K. Sun

doi:10.1117/12.600829

21 March 2005 Long-term in vivo harmonics imaging of zebrafish embryonic development based on a femtosecond Cr:forsterite laser

S.-Y. Chen, T.-H. Tsai, C.-S. Hsieh, S.-P. Tai, C.-Y. Lin, C.-Y. Ko, Y.-C. Chen, H.-J. Tsai, C.-H. Hu, C.-K. Sun

Author Affiliations +

Proceedings Volume 5714, Commercial and Biomedical Applications of Ultrafast Lasers V; (2005) https://doi.org/10.1117/12.600829
Event: Lasers and Applications in Science and Engineering, 2005, San Jose, California, United States

Abstract

Based on a femtosecond Cr:forsterite laser, harmonics optical microscopy (HOM) provides a truly “noninvasive” tool for in vivo and long-term study of vertebrate embryonic development. Based on optical nonlinearity, HOM provides sub-micrometer 3D spatial resolution and high 3D optical-sectioning power without using invasive and toxic fluorophores. Since only virtual-level-transition is involved, HOM is known to leave no energy deposition and no photodamage. Combined with second harmonic generation, which is sensitive to specific structure such as nerve and muscle fibers, HOM can perform functional studies of early developmental dynamics of many vertebrate physiological systems. Recently, zebrafish has become a standard model for many biological and medical studies of vertebrates, due to the similarity between embryonic development of zebrafish and human being. Here we demonstrate in vivo HOM studies of developmental dynamics of several important embryonic physiological systems in live zebrafish embryos, with focuses on the developments of brains, eyes, ears, and hearts. Based on a femtosecond Cr:forsterite laser, which provides the deepest penetration (~1.5mm) and least photodamage in the zebrafish embryo, complete developing processes of different physiological systems within a period of time longer than 20 hours can be non-invasively observed inside the same embryo.

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S.-Y. Chen, T.-H. Tsai, C.-S. Hsieh, S.-P. Tai, C.-Y. Lin, C.-Y. Ko, Y.-C. Chen, H.-J. Tsai, C.-H. Hu, and C.-K. Sun "Long-term in vivo harmonics imaging of zebrafish embryonic development based on a femtosecond Cr:forsterite laser", Proc. SPIE 5714, Commercial and Biomedical Applications of Ultrafast Lasers V, (21 March 2005); https://doi.org/10.1117/12.600829

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KEYWORDS

In vivo imaging

Second-harmonic generation

Femtosecond phenomena

Spindles

Tissues

Standards development

Microscopy

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