Experimental study of time-resolved measurements on turbid media: determination of optical properties and fluorescent inclusions characterization

Aurelie Laidevant; Anabela da Silva; Michel Berger; Jean-Marc Dinten

doi:10.1117/12.632877

7 October 2005 Experimental study of time-resolved measurements on turbid media: determination of optical properties and fluorescent inclusions characterization

Aurelie Laidevant, Anabela da Silva, Michel Berger, Jean-Marc Dinten

Author Affiliations +

Proceedings Volume 5859, Photon Migration and Diffuse-Light Imaging II; 58591F (2005) https://doi.org/10.1117/12.632877
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

Among the different existing techniques in optical molecular imaging, time-domain approaches can supply information on the probe concentration or localization, in case of specific fluorescent labelling. We present an experimental validation of an analytical solution to the time-domain fluorescence diffusion equation in an infinite medium. A fitting method issued from frequency domain calculations is also presented. The instrumentation employs a pulsed laser diode as light source, optical fibres, and a photomultiplier as the detector in a time correlated single photon counting (TCSPC) system. Measurements were performed on tissue simulating phantoms containing a small fluorescent inclusion, with the fibres either imbedded deep within the volume or placed at the surface, above the inclusion. Good adequacy was found between the simulations and the within medium measurements. We also demonstrate good performances of the method to recover the fluorophore localization.

Citation Download Citation

Aurelie Laidevant, Anabela da Silva, Michel Berger, and Jean-Marc Dinten "Experimental study of time-resolved measurements on turbid media: determination of optical properties and fluorescent inclusions characterization", Proc. SPIE 5859, Photon Migration and Diffuse-Light Imaging II, 58591F (7 October 2005); https://doi.org/10.1117/12.632877

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