Time resolved fluorescence imaging in diffuse media

Anand T. N. Kumar; Jesse Skoch; Frank L. Hammond III; Andrew K. Dunn; David A. Boas; Brian J. Bacskai

doi:10.1117/12.630990

22 November 2005 Time resolved fluorescence imaging in diffuse media

Anand T. N. Kumar, Jesse Skoch, Frank L. Hammond III, Andrew K. Dunn, David A. Boas, Brian J. Bacskai

Proceedings Volume 6009, Optical Methods in Drug Discovery and Development; 60090Y (2005) https://doi.org/10.1117/12.630990
Event: Optics East 2005, 2005, Boston, MA, United States

Abstract

We discuss the application of time domain fluorescence techniques to the recovery of targets embedded in several cm thick biological tissue. Considering the general time domain problem first, a singular value analysis is used to study the optimal use of multiple frequency components extracted from time domain data. Furthermore, a computationally efficient algorithm is presented to tomographically reconstruct fluorophore locations using their decay amplitudes and validated using phantom experiments. The reconstruction algorithm presented here has wide applicability for non-invasive, diagnostic fluorescence imaging in small animals and other biological systems, given that fluorescence lifetime is a sensitive indicator of local tissue environment and elementary interactions at the molecular level.

Citation Download Citation

Anand T. N. Kumar, Jesse Skoch, Frank L. Hammond III, Andrew K. Dunn, David A. Boas, and Brian J. Bacskai "Time resolved fluorescence imaging in diffuse media", Proc. SPIE 6009, Optical Methods in Drug Discovery and Development, 60090Y (22 November 2005); https://doi.org/10.1117/12.630990

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