Laser-dephosphorylation of phosphogelatin and its indirect quantitative analysis using FT-IR

Katsunori Ishii; Yoshihisa Ninomiya; Minori Takegoshi; Toshihiro Kushibiki; Sachiko Yoshihashi-Suzuki; Masaya Yamamoto; Yasuhiko Tabata; Kunio Awazu

doi:10.1117/12.642598

1 March 2006 Laser-dephosphorylation of phosphogelatin and its indirect quantitative analysis using FT-IR

Katsunori Ishii, Yoshihisa Ninomiya, Minori Takegoshi, Toshihiro Kushibiki, Sachiko Yoshihashi-Suzuki, Masaya Yamamoto, Yasuhiko Tabata, Kunio Awazu

Author Affiliations +

Proceedings Volume 6084, Optical Interactions with Tissue and Cells XVII; 608412 (2006) https://doi.org/10.1117/12.642598
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Phosphorylation and dephosphorylation are considered to be important reactions that control the active and inactive factors of proteins. In regenerative medicine of the osteoconnective tissue (a tendon, a ligament), it has been reported that the biomaterial possessing phosphate groups promote formation of HAP, the main component of hard tissues. The noncontact measurement of phosphate groups and low-destructive controlling of phosphate groups allow for the accurate regeneration of the osteoconnective tissue, and the validation. Our objective is to propose the nondestructive controlling and measuring method of phosphorylation for regenerative medicine. In this study, as the indirect quantitative analysis of phosphate groups, we examine the correlation between the mid-infrared absorbance ratio and the ratio of phosphate groups introduction theoretically calculated from a colorimetric determination method. And the noncontact controlling method of the quantities of phosphate groups, we examine the selective and low-destructive bond cutting of phosphate groups in the phosphogelatin using a mid-infrared laser.

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Katsunori Ishii, Yoshihisa Ninomiya, Minori Takegoshi, Toshihiro Kushibiki, Sachiko Yoshihashi-Suzuki, Masaya Yamamoto, Yasuhiko Tabata, and Kunio Awazu "Laser-dephosphorylation of phosphogelatin and its indirect quantitative analysis using FT-IR", Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 608412 (1 March 2006); https://doi.org/10.1117/12.642598

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KEYWORDS

Infrared radiation

Laser cutting

Mid-IR

Tissues

Absorption

Absorbance

FT-IR spectroscopy

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