Detection of 2-photon oxidation from a NIR laser using confocal microscopy

Kurt J. Schuster; Larry E. Estlack; Benjamin A. Rockwell; Michael L. Denton

doi:10.1117/12.644651

28 February 2006 Detection of 2-photon oxidation from a NIR laser using confocal microscopy

Kurt J. Schuster, Larry E. Estlack, Benjamin A. Rockwell, Michael L. Denton

Proceedings Volume 6084, Optical Interactions with Tissue and Cells XVII; 60841H (2006) https://doi.org/10.1117/12.644651
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Recent studies have determined that photochemical oxidation in cultured cells can be detected at peak irradiances as low as 8.5x10⁸ W cm^-2 (87-fs pulse). Fluorescent dyes, such as CM-H₂DCFDA, enable us to quantify the oxidation response of cells to mode-locked near-infrared (NIR) laser exposure. Using a modified confocal microscope, we characterize the time-dependent 2-photon induced fluorescence generated from a given NIR laser exposure. When cultured cells were then pre-loaded with antioxidants, ascorbic acid or N-acetyl-L-cysteine (NAC), they inhibit nonlinear oxidation with different efficiencies, providing insight regarding mechanisms of damage.

Citation Download Citation

Kurt J. Schuster, Larry E. Estlack, Benjamin A. Rockwell, and Michael L. Denton "Detection of 2-photon oxidation from a NIR laser using confocal microscopy", Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60841H (28 February 2006); https://doi.org/10.1117/12.644651

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