Detection of collagen by second harmonic microscopy as a diagnostic tool for liver fibrosis

Maruth Banavar; Eleanor P. W. Kable; Filip Braet; X. M. Wang; M. D. Gorrell; Guy Cox

doi:10.1117/12.649452

23 February 2006 Detection of collagen by second harmonic microscopy as a diagnostic tool for liver fibrosis

Maruth Banavar, Eleanor P. W. Kable, Filip Braet, X. M. Wang, M. D. Gorrell, Guy Cox

Proceedings Volume 6089, Multiphoton Microscopy in the Biomedical Sciences VI; 60891B (2006) https://doi.org/10.1117/12.649452
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Liver fibrosis has many causes, including hepatitis C, alcohol abuse, and non-alcoholic steatohepatitis. It is characterized by abnormal deposition of extracellular matrix proteins, mainly collagen. The deposition of these proteins results in impaired liver function caused by distortion of the hepatic architecture by fibrous scar tissue. The unique triple helix structure of collagen and high level of crystallinity make it very efficient for generating second harmonic signals. In this study we have set out to see if second harmonic imaging of collagen can be used as a non-biased quantitative tool for classification of fibrosis levels in liver biopsies and if it can detect early fibrosis formation not detected by current methods.

Citation Download Citation

Maruth Banavar, Eleanor P. W. Kable, Filip Braet, X. M. Wang, M. D. Gorrell, and Guy Cox "Detection of collagen by second harmonic microscopy as a diagnostic tool for liver fibrosis", Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 60891B (23 February 2006); https://doi.org/10.1117/12.649452

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available