Imaging of eye tumor in the mouse model of retinoblastoma with spectral-domain optical coherence tomography

Shuliang Jiao; Marco Ruggeri; Hassan Wehbe; Giovanni Gregory; Maria E. Jockovich; Abigail Hackam; Carmen A. Puliafito M.D.

doi:10.1117/12.700338

5 March 2007 Imaging of eye tumor in the mouse model of retinoblastoma with spectral-domain optical coherence tomography

Shuliang Jiao, Marco Ruggeri, Hassan Wehbe, Giovanni Gregory, Maria E. Jockovich, Abigail Hackam, Carmen A. Puliafito M.D.

Author Affiliations +

Proceedings Volume 6426, Ophthalmic Technologies XVII; 64260D (2007) https://doi.org/10.1117/12.700338
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Noninvasive in vivo examination of the rodent retina without sacrificing the animal is the key to being able to perform longitudinal studies. This allows the monitoring of disease progression and the response to therapies through its entire course in individual animal. A high-speed high resolution three-dimensional spectral-domain OCT is built for non-contact in vivo imaging of rodent retina. The system is able to acquire high quality 3D images of the rodent retina in 2.7 seconds (total imaging time is ~5 minutes). The system was tested on mice with normal retina (B6/SJLF2), mouse model for photoreceptor degeneration (Rho^-/-), and mouse model for retinoblastoma (LH_BETAT_AG). For the first time to our knowledge, 3D image of the tumor in retinoblastoma mouse model was successfully imaged in vivo. By segmenting the tumor boundaries in each frame of the OCT image the volume of the tumor was successfully calculated.

Citation Download Citation

Shuliang Jiao, Marco Ruggeri, Hassan Wehbe, Giovanni Gregory, Maria E. Jockovich, Abigail Hackam, and Carmen A. Puliafito M.D. "Imaging of eye tumor in the mouse model of retinoblastoma with spectral-domain optical coherence tomography", Proc. SPIE 6426, Ophthalmic Technologies XVII, 64260D (5 March 2007); https://doi.org/10.1117/12.700338

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