Sensitivity and robustness of methods for analyzing time-resolved fluorescence measurements of layered biological tissue

Anant Agrawal; Camisha Parker; Tariq Qazi; Krishan Agrawal; Joshua Pfefer

doi:10.1117/12.717654

6 February 2007 Sensitivity and robustness of methods for analyzing time-resolved fluorescence measurements of layered biological tissue

Anant Agrawal, Camisha Parker, Tariq Qazi, Krishan Agrawal, Joshua Pfefer

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Proceedings Volume 6430, Advanced Biomedical and Clinical Diagnostic Systems V; 643022 (2007) https://doi.org/10.1117/12.717654
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Time-resolved fluorescence (TRF) measurements from layered biological tissue provide chemical and structural information which may be useful for imaging or single-point tissue diagnostics. While several techniques for analyzing TRF data have been proposed in the literature, a rigorous theoretical evaluation of these approaches has not been performed. In this study we have evaluated the sensitivity and robustness of four methods for analyzing TRF signals: biexponential deconvolution, single exponential deconvolution, Laguerre deconvolution, and direct peak width computation. Each of these analyses was performed on a large dataset of synthetic fluorescence decay curves. Each decay curve was generated by numerically convolving a pre-recorded nitrogen laser pulse with a biexponential decay based on fluorescence lifetimes of colonic mucosa. The relative contribution of each mucosal layer to the total TRF signal as well as the superficial layer's inherent lifetime were varied so as to investigate sensitivity to morphological and biochemical changes representative of a neoplastic disease process. To evaluate robustness, pre-set levels of Gaussian-distributed noise were added to the convolved curves to achieve variations in the signal-to-noise ratio. The relative merits and pitfalls of each analytical method are discussed.

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Anant Agrawal, Camisha Parker, Tariq Qazi, Krishan Agrawal, and Joshua Pfefer "Sensitivity and robustness of methods for analyzing time-resolved fluorescence measurements of layered biological tissue", Proc. SPIE 6430, Advanced Biomedical and Clinical Diagnostic Systems V, 643022 (6 February 2007); https://doi.org/10.1117/12.717654

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KEYWORDS

Luminescence

Tissues

Deconvolution

Time resolved spectroscopy

Biological research

Testing and analysis

Diagnostics

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