Rotational behavior of erythrocytes in optical trap: revisited by confocal fluorescence microscopy

Khyati Mohanty; Samarendra Mohanty; Shamci Monajembashi; Karl Otto Greulich

doi:10.1117/12.703320

19 February 2007 Rotational behavior of erythrocytes in optical trap: revisited by confocal fluorescence microscopy

Khyati Mohanty, Samarendra Mohanty, Shamci Monajembashi, Karl Otto Greulich

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 644111 (2007) https://doi.org/10.1117/12.703320
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

There has been considerable current interest in rotational behavior of red blood cells (RBC) in optical tweezers. However, the mechanism of rotation in polarized tweezers is still not well understood and there exists conflicts in the understanding of this phenomenon. Therefore, we re-examined the underlying phenomenon by use of confocal fluorescence microscopy. Under different osmolarities of the buffer, the three dimensionally reconstructed images showed that the trapped RBC maintains its discotic shape and is oriented in vertical direction. Using dual optical tweezers, the RBC could also be oriented three-dimensionally in a controlled manner. Since, no folding of the RBC was observed under optical trapping beam, the rotational mechanism based on optical birefringence caused by folding of RBC can be ruled out. The alignment of RBC with polarization of the tweezers beam can be attributed to its formbirefringence. We also present the mechanism for possible rotational behavior of RBC in circularly polarized beam.

Citation Download Citation

Khyati Mohanty, Samarendra Mohanty, Shamci Monajembashi, and Karl Otto Greulich "Rotational behavior of erythrocytes in optical trap: revisited by confocal fluorescence microscopy", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 644111 (19 February 2007); https://doi.org/10.1117/12.703320

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KEYWORDS

Optical tweezers

Confocal microscopy

Luminescence

Dielectric polarization

Polarization

Birefringence

Microscopy

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