HiFLO: a high-throughput system for spatial analysis of FISH loci in interphase nuclei

Prabhakar R. Gudla; J. Collins; K. J. Meaburn; T. Misteli; S. J. Lockett

doi:10.1117/12.700817

19 February 2007 HiFLO: a high-throughput system for spatial analysis of FISH loci in interphase nuclei

Prabhakar R. Gudla, J. Collins, K. J. Meaburn, T. Misteli, S. J. Lockett

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 644119 (2007) https://doi.org/10.1117/12.700817
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Numerous investigations in the last years focused on chromosome and gene arrangements through the application of statistical methods that analyze the non randomness of spatial distributions of fluorescence in situ hybridization (FISH) labeled nucleic acid sequences in terms of their distance to the nuclear centers and their proximity to each other. However, existing imaging processing methods are rather limited in extracting sufficient number of nuclei with FISH label sequences, and manual analysis is unreasonably time-consuming and subjective. This paper presents an automated system that integrates a series of advanced image processing methods to over come this rate-limiting step. Evaluation results show that the proposed method is efficient, robust, and effective in extracting individual nuclei with FISH labels.

Citation Download Citation

Prabhakar R. Gudla, J. Collins, K. J. Meaburn, T. Misteli, and S. J. Lockett "HiFLO: a high-throughput system for spatial analysis of FISH loci in interphase nuclei", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 644119 (19 February 2007); https://doi.org/10.1117/12.700817

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