Full-field OCT for developmental biology

Rui Wang; Ping Xue; Wenjia Bai; Dieyan Chen

doi:10.1117/12.741591

1 May 2007 Full-field OCT for developmental biology

Rui Wang, Ping Xue, Wenjia Bai, Dieyan Chen

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65341U (2007) https://doi.org/10.1117/12.741591
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

In this paper, we developed a full-field OCT system using thermal light as the low-coherence light source. A well-known Linnik interferometer configuration was used. Broad spectral width of the thermal light 450-650nm was used to achieve high axial resolution of 1.1&mgr;m in biological sample for OCT imaging. Two water immersion objectives of 0.5N.A were used to balance the dispersion and a transverse resolution of 0.7μm was obtained. With a fast machine-coding algorithm, system sensitivity of more than 80dB and imaging rate as high as 18frame/s with 500x500 pixels per frame could be achieved. Mouse embryos were imaged in vivo with full-field OCT at different depth for the developmental study. Useful information for pre-implementation genetic diagnosis (PGD) was obtained by image analysis and segmentation. As far as known, for the fist time, 3D images of mammalian embryos were obtained with full-field OCT without the need of dye labeling.

Citation Download Citation

Rui Wang, Ping Xue, Wenjia Bai, and Dieyan Chen "Full-field OCT for developmental biology", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341U (1 May 2007); https://doi.org/10.1117/12.741591

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