In vivo measurement of the carotenoid level using portable resonance Raman spectroscopy

Yonghong Shao; Junle Qu D.D.S.; Yonghong He

doi:10.1117/12.726859

19 July 2007 In vivo measurement of the carotenoid level using portable resonance Raman spectroscopy

Yonghong Shao, Junle Qu D.D.S., Yonghong He

Proceedings Volume 6628, Diagnostic Optical Spectroscopy in Biomedicine IV; 662817 (2007) https://doi.org/10.1117/12.726859
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Carotenoid is an important antioxidant in human body, which can eliminate the free radicals and other harmful reactive oxygen species. The standard technique for measuring carotenoid is high-pressure liquid chromatography which involves using chemicals and is invasive. In this paper, we present a portable resonance Raman spectroscopy system for measuring carotenoid in vivo, which is noninvasive, highly sensitive and compact. A small diode-pumped all solid-state 473nm laser instead of a 488nm Argon ion laser is used to excite in vivo the carotenoid in the thumb, and the resonance Raman scattering light intensity is measured to assess the carotenoid level. Basically, it is difficult to detect the very weak resonance Raman scattering light because it is overlapping with the strong fluorescence. Our investigation shows that matching glycerol can help to reduce tissue scattering and increase light collecting efficiency. We demonstrate that the employment of optical matching technology for measuring carotenoid resonance Raman spectra in tissue can improve the signal-to-noise ratio by 3.9dB.

Citation Download Citation

Yonghong Shao, Junle Qu D.D.S., and Yonghong He "In vivo measurement of the carotenoid level using portable resonance Raman spectroscopy", Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 662817 (19 July 2007); https://doi.org/10.1117/12.726859

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available