Dynamic in vivo analysis of drug induced actin cytoskeleton degradation by digital holographic microscopy

Juergen Schnekenburger; Ilona Bredebusch; Patrik Langehanenberg; Wolfram Domschke; Gert von Bally; Björn Kemper

doi:10.1117/12.728557

13 July 2007 Dynamic in vivo analysis of drug induced actin cytoskeleton degradation by digital holographic microscopy

Juergen Schnekenburger, Ilona Bredebusch, Patrik Langehanenberg, Wolfram Domschke, Gert von Bally, Björn Kemper

Proceedings Volume 6633, Biophotonics 2007: Optics in Life Science; 66330G (2007) https://doi.org/10.1117/12.728557
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

The actin cytoskeleton mediates a variety of crucial cellular functions as migration, intracellular transport, exocytosis, endocytosis and force generation. The highly dynamic actin fibers are therefore targets for several drugs and toxins. However the study of actin interfering processes by standard microscopy techniques fails in the detailed resolution of dynamic spatial alterations required for a deeper understanding of toxic effects. Here we applied digital holographic microscopy in the online functional analysis of the actin cytoskeleton disrupting marine toxin Latrunculin B. SEM and fluorescence microscopy showed rapid Latrunculin B induced alterations in cell morphology and actin fiber degradation in pancreas tumor cells. The dynamic digital holographic in vivo analysis of the drug dependent cellular processes demonstrated differences in the actin cytoskeleton stability of highly differentiated and dedifferentiated pancreas tumor cell lines. The spatial resolution of the morphological alterations revealed unequal changes in cell morphology. While cells with a low metastatic potential showed Latrunculin B induced cell collapse within 4 h the metastatic tumor cells were increased in cell volume indicating Latrunculin B effects also on cell water content. These data demonstrate that marker free, non-destructive online analysis of cellular morphology and dynamic spatial processes in living cells by digital holography offers new insights in actin dependent cellular mechanisms. Digital holographic microscopy was shown to be a versatile tool in the screening of toxic drug effects and cancer cell biology.

Citation Download Citation

Juergen Schnekenburger, Ilona Bredebusch, Patrik Langehanenberg, Wolfram Domschke, Gert von Bally, and Björn Kemper "Dynamic in vivo analysis of drug induced actin cytoskeleton degradation by digital holographic microscopy", Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66330G (13 July 2007); https://doi.org/10.1117/12.728557

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