Influence of measurement geometry on the human skin reflectance spectra detection

E. Borisova; P. Troyanova M.D.; L. Avramov

doi:10.1117/12.753142

1 August 2007 Influence of measurement geometry on the human skin reflectance spectra detection

E. Borisova, P. Troyanova M.D., L. Avramov

Proceedings Volume 6734, International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine; 673416 (2007) https://doi.org/10.1117/12.753142
Event: International Conference on Lasers, Applications, and Technologies '07, 2007, Minsk, Belarus

Abstract

One of the optical techniques applied in skin lesion investigations is reflectance spectroscopy of cutaneous tissues. Diffuse reflectance signals could be applied for absolute determination of absorption and scattering coefficients of biological tissue and give reliable results for wide range of wavelengths. However, light distribution anisotropy leads to significant influence over the reflectance spectra appearance depending on the used geometry. To obtain good reproducibility and repeatability of the results, one needs to fix carefully the geometrical conditions. We present skin reflectance spectra obtained at different angles and distances from tissue surface and discuss possible reasons for differences observed. In the case of small angles of incidence, we have significant losses of the backscattered light related to the anisotropy of the tissue and the intensity observed of the reflected signal is much lower than in the case of diffuse reflectance signal detected at higher angles. In the case of direct contact between skin surface and end tip of the fibers great reduction of the short wavelength component is observed. The appearance of higher intensity blue signal as the distance between end tip of the optical fibers and skin surface is increased could be related to the specular component of the reflectance spectra. An optimum distance exists where the registered signal has the greatest value, which is related to the specific parameters of the optical fibers used, such as diameter and optical aperture.

Citation Download Citation

E. Borisova, P. Troyanova M.D., and L. Avramov "Influence of measurement geometry on the human skin reflectance spectra detection", Proc. SPIE 6734, International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine, 673416 (1 August 2007); https://doi.org/10.1117/12.753142

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