The optical biomedical sensors for DNA detection and imaging based on two-photon excited luminescent styryl dyes: phototoxic influence on the DNA

Valeriy M. Yashchuk; Vladislav Yu. Kudrya; Mykhaylo Yu. Losytskyy; Valentyna P. Tokar; Sergiy M. Yarmoluk; Igor M. Dmytruk; Vadym M. Prokopets; Vladyslava B. Kovalska; Anatoliy O. Balanda; Dmytro V. Kryvorotenko; Tymish Yu. Ogul'chansky

doi:10.1117/12.778096

8 November 2007 The optical biomedical sensors for DNA detection and imaging based on two-photon excited luminescent styryl dyes: phototoxic influence on the DNA

Valeriy M. Yashchuk, Vladislav Yu. Kudrya, Mykhaylo Yu. Losytskyy, Valentyna P. Tokar, Sergiy M. Yarmoluk, Igor M. Dmytruk, Vadym M. Prokopets, Vladyslava B. Kovalska, Anatoliy O. Balanda, Dmytro V. Kryvorotenko, Tymish Yu. Ogul'chansky

Author Affiliations +

Proceedings Volume 6796, Photonics North 2007; 67960M (2007) https://doi.org/10.1117/12.778096
Event: Photonics North 2007, 2007, Ottawa, Canada

Abstract

The optical absorption, fluorescence and phosphorescence of the novel styryl dyes developed for the fluorescent detection of DNA were investigated. The energy structures of dye molecules as well as spectral manifestations of the dyes aggregate formation and interaction with DNA were studied. The dramatic increase (up to 1000 times) of the fluorescence intensity of dyes in the presence of DNA was observed. The photostability and phototoxic influence on the DNA of several styryl dyes were studied by analyzing absorption, fluorescence and phosphorescence spectra of these dyes and dye-DNA systems. Changes of the optical density value of dye-DNA solutions caused by the visible light irradiation were fixed in the wavelength regions of the DNA absorption and of the dye absorption. Fluorescence emission of dye-DNA complexes upon two-photon excitation (TPE) at wavelength 1064 nm with the 20 ns pulsed YAG: Nd3+ laser and at 840 nm with the 90 fs pulsed Ti:sapphire laser was registered. The values of two-photon absorption cross-sections of dye-DNA complexes were evaluated.

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Valeriy M. Yashchuk, Vladislav Yu. Kudrya, Mykhaylo Yu. Losytskyy, Valentyna P. Tokar, Sergiy M. Yarmoluk, Igor M. Dmytruk, Vadym M. Prokopets, Vladyslava B. Kovalska, Anatoliy O. Balanda, Dmytro V. Kryvorotenko, and Tymish Yu. Ogul'chansky "The optical biomedical sensors for DNA detection and imaging based on two-photon excited luminescent styryl dyes: phototoxic influence on the DNA", Proc. SPIE 6796, Photonics North 2007, 67960M (8 November 2007); https://doi.org/10.1117/12.778096

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