Alpha-fetoprotein detection by using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Ying-Feng Chang; Ran-Chou Chen; Ying-Chang Li; Chih-Jen Yu; Bao-Yu Hsieh; Chien Chou

doi:10.1117/12.754617

8 January 2008 Alpha-fetoprotein detection by using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Ying-Feng Chang, Ran-Chou Chen, Ying-Chang Li, Chih-Jen Yu, Bao-Yu Hsieh, Chien Chou

Author Affiliations +

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 68261B (2008) https://doi.org/10.1117/12.754617
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

Alpha-fetoprotein (AFP) detection by using a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor is setup and experimentally demonstrated. It is based on gold nanoparticle (GNP) and coupled with localized surface plasmon wave on the surface of GNP. In this experiment, the fluorophores are labeled on anti-AFP which are bound to protein A conjugated GNP. Thus, LSPCF is excited with high efficiency in the near field of localized surface plasmon wave. Therefore, not only the sensitivity of LSPCF biosensor is enhanced but also the specific selectivity of AFP is improved. Experimentally, the ability of real time measurement in the range of AFP concentration from 0.1ng/ml to 100ng/ml was detected. To compare with conventional methods such as enzyme-linked immunosorbent assay (ELISA) or radioimmunoassay (RIA), the LSPCF fiber-optic biosensor performs higher or comparable detection sensitivity, respectively.

Citation Download Citation

Ying-Feng Chang, Ran-Chou Chen, Ying-Chang Li, Chih-Jen Yu, Bao-Yu Hsieh, and Chien Chou "Alpha-fetoprotein detection by using a localized surface plasmon coupled fluorescence fiber-optic biosensor", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68261B (8 January 2008); https://doi.org/10.1117/12.754617

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