Signal sources in elastic light scattering by biological cells and tissues: what can elastic light scattering spectroscopy tell us?

M. Xu; Tao T. Wu; Jianan Y. Qu

doi:10.1117/12.762368

22 February 2008 Signal sources in elastic light scattering by biological cells and tissues: what can elastic light scattering spectroscopy tell us?

M. Xu, Tao T. Wu, Jianan Y. Qu

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Proceedings Volume 6864, Biomedical Applications of Light Scattering II; 68640Y (2008) https://doi.org/10.1117/12.762368
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We used a unified Mie and fractal model to analyze elastic light spectroscopy of cell suspensions to obtain the size distributions of cells and nuclei, their refractive indices, and the background refractive index fluctuation inside the cell, for different types of cells, including human cervical squamous carcinoma epithelial (SiHa) cells, androgen-independent malignant rat prostate carcinoma epithelial (AT3.1) cells, non-tumorigenic fibroblast (Rat1p) cells in the plateau phase of growth, and tumorigenic fibroblast (Rat1-T1E) cells in the exponential phase of growth. Signal sources contributing to the scattering (μs) and reduced scattering (μ^'s) coefficients for these cells of various types or at different growth stages are compared. It is shown that the contribution to μ_s from the nucleus is much more important than that from the background refractive index fluctuation. This trend is more significant with increase of the probing wavelength. On the other hand, the background refractive index fluctuation overtakes the nucleus and may even dominate in the contribution to reduced scattering. The implications of the above findings on biomedical light scattering techniques are discussed.

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M. Xu, Tao T. Wu, and Jianan Y. Qu "Signal sources in elastic light scattering by biological cells and tissues: what can elastic light scattering spectroscopy tell us?", Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 68640Y (22 February 2008); https://doi.org/10.1117/12.762368

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KEYWORDS

Light scattering

Scattering

Refractive index

Tumor growth modeling

Tissues

Fractal analysis

Spectroscopy

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