Photobleaching behavior of protoporphyrin IX during 5-aminolevulinic acid marked glioblastoma detection

Neda Haj-Hosseini; Johan Richter; Stefan Andersson-Engels; Karin Wårdell

doi:10.1117/12.808156

23 February 2009 Photobleaching behavior of protoporphyrin IX during 5-aminolevulinic acid marked glioblastoma detection

Neda Haj-Hosseini, Johan Richter, Stefan Andersson-Engels, Karin Wårdell

Author Affiliations +

Proceedings Volume 7161, Photonic Therapeutics and Diagnostics V; 716131 (2009) https://doi.org/10.1117/12.808156
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

The highly malignant brain tumor, glioblastoma multiforme (GBM), is difficult to fully delineate during surgical resection due to its infiltrative ingrowth and morphological similarities to surrounding functioning brain tissue. Selectiveness of GBM to 5-aminolevulinic acid (5-ALA) induced protoporphyrin IX (PpIX) is reported by other researchers to visualize tumor margins under blue light microscopy. To allow objective detection of GBM, a compact and portable fiber optic based fluorescence spectroscopy system is developed. This system is able to deliver excitation laser light (405 nm) in both the continuous and pulsed mode. PpIX fluorescence peaks are detected at 635 and 704 nm, using a fiber-coupled spectrometer. It is necessary to optimize the detection efficiency of the system as the PpIX quickly photobleaches during the laser illumination. A light dose of 2.5 mJ (fluence rate = 9 mJ/mm²) is experimentally approved to excite an acceptable level of fluourescence signal arising from glioblastoma. In pulsed illumination mode, an excitation dose of 2.5 mJ, with a dark interval of 0.5 s (duty cycle 50%) shows a significantly shorter photobleaching time in comparison to the continuous illumination mode with the same laser power (p < 0.05). To avoid photobleaching (the remaining signal is more than 90% of its initial value) when measuring with 2.5 mJ delivered energy, the time for continuous and pulsed illumination should be restricted to 2.5 and 1.1 s, respectively.

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Neda Haj-Hosseini, Johan Richter, Stefan Andersson-Engels, and Karin Wårdell "Photobleaching behavior of protoporphyrin IX during 5-aminolevulinic acid marked glioblastoma detection", Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 716131 (23 February 2009); https://doi.org/10.1117/12.808156

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