Ultrasound-modulated fluorescence based on a fluorophore-quencher labeled microbubble system

Baohong Yuan

doi:10.1117/12.809847

24 February 2009 Ultrasound-modulated fluorescence based on a fluorophore-quencher labeled microbubble system

Baohong Yuan

Proceedings Volume 7177, Photons Plus Ultrasound: Imaging and Sensing 2009; 71771J (2009) https://doi.org/10.1117/12.809847
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Ultrasound-modulated fluorescence from a fluorophore-quencher labeled microbubble system driven by a single ultrasound pulse was theoretically quantified by solving a modified Herring equation (for bubble oscillation), a two-energy-level rate equation (for fluorophore excitation), and a diffusion equation (for light propagation in tissue). The efficiency of quenching caused by fluorescence resonance energy transfer (FRET) between the fluorophore and the quencher was modulated when the microbubble oscillates in size driven by the ultrasound pulse. Both intensity- and lifetime-based imaging methods are discussed. An important finding in this study is that ultrasound-modulated fluorescent photons may be temporally separated from the un-modulated fluorescent photons if a super-short laser pulse is adopted. This result implies that the modulation efficiency may only be limited by thermal noise of the measurement system.

Citation Download Citation

Baohong Yuan "Ultrasound-modulated fluorescence based on a fluorophore-quencher labeled microbubble system", Proc. SPIE 7177, Photons Plus Ultrasound: Imaging and Sensing 2009, 71771J (24 February 2009); https://doi.org/10.1117/12.809847

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