Detection of melanoma cells suspended in mononuclear cells and blood plasma using photoacoustic generation

Emily M. Spradling; John A. Viator

doi:10.1117/12.809859

12 February 2009 Detection of melanoma cells suspended in mononuclear cells and blood plasma using photoacoustic generation

Emily M. Spradling, John A. Viator

Author Affiliations +

Proceedings Volume 7177, Photons Plus Ultrasound: Imaging and Sensing 2009; 71771Z (2009) https://doi.org/10.1117/12.809859
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Melanoma is the deadliest form of skin cancer. Although the initial malignant cells are removed, it is impossible to determine whether or not the cancer has metastasized until a secondary tumor forms that is large enough to detect with conventional imaging. Photoacoustic detection of circulating melanoma cells in the bloodstream has shown promise for early detection of metastasis that may aid in treatment of this aggressive cancer. When blood is irradiated with energy from an Nd:YAG laser at 532 nm, photoacoustic signals are created and melanoma cells can be differentiated from the surrounding cells based on waveforms produced by an oscilloscope. Before this can be used as a diagnostic technique, however, we needed to investigate several parameters. Specifically, the current technique involves the in vitro separation of blood through centrifugation to isolate and test only the white blood cell layer. Using this method, we have detected a single cultured melanoma cell among a suspension of white blood cells. However, the process could be made simpler if the plasma layer were used for detection instead of the white blood cell layer. This layer is easier to obtain after blood separation, the optical difference between plasma and melanoma cells is more pronounced in this layer than in the white blood cell layer, and the possibility that any stray red blood cells could distort the results is eliminated. Using the photoacoustic apparatus, we detected no melanoma cells within the plasma of whole blood samples spiked with cultured melanoma cells.

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Emily M. Spradling and John A. Viator "Detection of melanoma cells suspended in mononuclear cells and blood plasma using photoacoustic generation", Proc. SPIE 7177, Photons Plus Ultrasound: Imaging and Sensing 2009, 71771Z (12 February 2009); https://doi.org/10.1117/12.809859

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KEYWORDS

Blood

Melanoma

Plasma

Cancer

Photoacoustic spectroscopy

Tumors

Oscilloscopes

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