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13 February 2009 Multicolor fluctuation spectroscopy in cells
Michelle A. Digman, Enrico Gratton
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Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 718303 (2009) https://doi.org/10.1117/12.814875
Event: SPIE BiOS, 2009, San Jose, California, United States
Abstract
Based on the Fluctuation Correlation principle we have developed a method that is capable of measuring the stoichiometry of molecular complexes and mapping dynamic processes in living cells. The method is based on measuring simultaneously fluctuations of the fluorescence intensity at two image channels, each detecting a different kind of protein. This is an extension of the number and brightness analysis in which we use the use of the ratio between the variance and the average intensity to obtain the brightness of molecules.
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Michelle A. Digman and Enrico Gratton "Multicolor fluctuation spectroscopy in cells", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 718303 (13 February 2009); https://doi.org/10.1117/12.814875
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KEYWORDS
Fluorescence correlation spectroscopy
Diffusion
Proteins
Molecules
Particles
Microscopes
Luminescence
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