Three-dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation

Daekeun Kim; Peter T. C. So

doi:10.1117/12.810910

13 February 2009 Three-dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation

Daekeun Kim, Peter T. C. So

Author Affiliations +

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71831B (2009) https://doi.org/10.1117/12.810910
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Laser scanning systems for two-photon microscopy and fabrication have been proven to be excellent in depth-resolving capability for years. However, their applications have been limited to laboratory use due to their intrinsic slow nature. The recently introduced temporal focusing concept enables wide-field optical sectioning and thus has potential in both high-speed 3D imaging and 3D mass-production fields. In this paper, we use the ultrafast optical pulse manipulation to generate two-photon excitation depth-resolved wide-field illumination (TPEDRWFI). The design parameters for the illumination were chosen based on numerical simulation of the temporal focusing. The imaging system was implemented, and the optical sectioning performance was compared with experimental result.

Citation Download Citation

Daekeun Kim and Peter T. C. So "Three-dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831B (13 February 2009); https://doi.org/10.1117/12.810910

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available