3D microscopy with a double-helix point spread function

Sri Rama Prasanna Pavani; Rafael Piestun

doi:10.1117/12.816585

3 March 2009 3D microscopy with a double-helix point spread function

Sri Rama Prasanna Pavani, Rafael Piestun

Proceedings Volume 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI; 71840I (2009) https://doi.org/10.1117/12.816585
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Double-helix point spread function (DH-PSF) is an engineered three-dimensional (3D) PSF specifically designed for 3D position estimation and imaging. It exhibits two lobes that rotate continuously around the optical axis with propagation. An information theoretical analysis shows that the DH-PSF carries higher and more uniform Fisher Information than the standard PSF through the 3D volume of interest. Experiments with DH-PSF demonstrate nanometer scale position localization accuracies in all three dimensions. Further, a variety of microscopy techniques such as bright-field, dark-field, and fluorescence can be directly transformed into their DH-PSF counterparts by placing an appropriate phase mask in the imaging path. The flexibility and performance make the DH-PSF attractive for 3D position localization in both photon-limited and photon-unlimited regimes.

Citation Download Citation

Sri Rama Prasanna Pavani and Rafael Piestun "3D microscopy with a double-helix point spread function", Proc. SPIE 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, 71840I (3 March 2009); https://doi.org/10.1117/12.816585

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