Spatially resolved bimodal spectroscopy for classification/evaluation of mouse skin inflammatory and pre-cancerous stages

Gilberto Díaz-Ayil; Marine Amouroux; Fabien Clanché; Yves Granjon; Walter C. P. M. Blondel

doi:10.1117/12.831628

7 July 2009 Spatially resolved bimodal spectroscopy for classification/evaluation of mouse skin inflammatory and pre-cancerous stages

Gilberto Díaz-Ayil, Marine Amouroux, Fabien Clanché, Yves Granjon, Walter C. P. M. Blondel

Author Affiliations +

Proceedings Volume 7368, Clinical and Biomedical Spectroscopy; 73680F (2009) https://doi.org/10.1117/12.831628
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Spatially-resolved bimodal spectroscopy (multiple AutoFluorescence AF excitation and Diffuse Reflectance DR), was used in vivo to discriminate various healthy and precancerous skin stages in a pre-clinical model (UV-irradiated mouse): Compensatory Hyperplasia CH, Atypical Hyperplasia AH and Dysplasia D. A specific data preprocessing scheme was applied to intensity spectra (filtering, spectral correction and intensity normalization), and several sets of spectral characteristics were automatically extracted and selected based on their discrimination power, statistically tested for every pair-wise comparison of histological classes. Data reduction with Principal Components Analysis (PCA) was performed and 3 classification methods were implemented (k-NN, LDA and SVM), in order to compare diagnostic performance of each method. Diagnostic performance was studied and assessed in terms of Sensibility (Se) and Specificity (Sp) as a function of the selected features, of the combinations of 3 different inter-fibres distances and of the numbers of principal components, such that: Se and Sp ≈ 100% when discriminating CH vs. others; Sp ≈ 100% and Se > 95% when discriminating Healthy vs. AH or D; Sp ≈ 74% and Se ≈ 63% for AH vs. D.

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Gilberto Díaz-Ayil, Marine Amouroux, Fabien Clanché, Yves Granjon, and Walter C. P. M. Blondel "Spatially resolved bimodal spectroscopy for classification/evaluation of mouse skin inflammatory and pre-cancerous stages", Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 73680F (7 July 2009); https://doi.org/10.1117/12.831628

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KEYWORDS

Atrial fibrillation

Spectroscopy

Skin

Selenium

Diffuse reflectance spectroscopy

Optical filters

Tissues

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