Anti-tumor effects on the combination of photodynamic therapy with arsenic compound in TC-1 cells implanted C57BL/6 mice

Kyu Wan Lee; Lan Ying Wen; Su Mi Bae; Choong Hak Park; Woo Kyu Jeon; Doo Yun Lee; Woong Shick Ahn

doi:10.1117/12.824239

13 July 2009 Anti-tumor effects on the combination of photodynamic therapy with arsenic compound in TC-1 cells implanted C57BL/₆ mice

Kyu Wan Lee, Lan Ying Wen, Su Mi Bae, Choong Hak Park, Woo Kyu Jeon, Doo Yun Lee, Woong Shick Ahn

Author Affiliations +

Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 738041 (2009) https://doi.org/10.1117/12.824239
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

The effects of As₄O₆ were studied as adjuvant on photodynamic therapy. As₄O₆ is considered to have anticancer activity via several biological actions such as free radical producing and inhibition of VEGF expression. In vitro experiments, cell proliferation and morphology were determined by MTT assay. Also, quantitative PCR array was performed to study the synergetic mechanism. Additionally, this study was supported by the finding that combination of photodynamic therapy and As₄O₆ shows an inhibition effect of tumor growth in C57BL/6 mice with TC-1 cells xenographs in vivo. Radachlorin and As₄O₆ significantly inhibited TC-1 cell proliferation in a dose-dependent manner (P < 0.05). Antiproliferative effect of combination treatment was significantly higher than those of TC-1 cells treated with either photodynamic therapy or As₄O₆ (62.4 and 52.5% decrease, respectively, compared to photodynamic therapy or As₄O₆ alone, P < 0.05). In addition, cell proliferation in combination of photodynamic therapy and As₄O₆ treatment significantly decreased by 77.4% compared to vehicle-only treated TC-1 cells (P < 0.05). Cell survival pathway (Naip1, Tert and Aip1) and p53-dependent pathway (Bax, p21^Cip1, Fas, Gadd₄5, IGFBP-3 and Mdm-2) were markedly increased by combination treatment of photodynamic therapy and As₄O₆. Besides, the immunology response NEAT pathway (Ly- 12, CD178 and IL-2) also modulated after combination treatment of photodynamic therapy and As₄O₆. This combination effect apparently shows a same pattern in vivo model. These findings suggest the benefit of the combination treatment of photodynamic therapy and As₄O₆ for the inhibition of cervical cancer growth.

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Kyu Wan Lee, Lan Ying Wen, Su Mi Bae, Choong Hak Park, Woo Kyu Jeon, Doo Yun Lee, and Woong Shick Ahn "Anti-tumor effects on the combination of photodynamic therapy with arsenic compound in TC-1 cells implanted C57BL/₆ mice", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 738041 (13 July 2009); https://doi.org/10.1117/12.824239

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