A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions

Michael S. D. Smith; Alex C. T. Ko; Andrew Ridsdale; Bernie Schattka; Adrian Pegoraro; Mark D. Hewko; Masashi Shiomi; Albert Stolow; Michael G. Sowa

doi:10.1117/12.840500

4 August 2009 A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions

Michael S. D. Smith, Alex C. T. Ko, Andrew Ridsdale, Bernie Schattka, Adrian Pegoraro, Mark D. Hewko, Masashi Shiomi, Albert Stolow, Michael G. Sowa

Author Affiliations +

Proceedings Volume 7386, Photonics North 2009; 73860I (2009) https://doi.org/10.1117/12.840500
Event: Photonics North 2009, 2009, Quebec, Canada

Abstract

In this study we compare the single-photon autofluorescence and multi-photon emission spectra obtained from the luminal surface of healthy segments of artery with segments where there are early atherosclerotic lesions. Arterial tissue was harvested from atherosclerosis-prone WHHL-MI rabbits (Watanabe heritable hyperlipidemic rabbit-myocardial infarction), an animal model which mimics spontaneous myocardial infarction in humans. Single photon fluorescence emission spectra of samples were acquired using a simple spectrofluorometer set-up with 400 nm excitation. Samples were also investigated using a home built multi-photon microscope based on a Ti:sapphire femto-second oscillator. The excitation wavelength was set at 800 nm with a ~100 femto-second pulse width. Epi-multi-photon spectroscopic signals were collected through a fibre-optics coupled spectrometer. While the single-photon fluorescence spectra of atherosclerotic lesions show minimal spectroscopic difference from those of healthy arterial tissue, the multi-photon spectra collected from atherosclerotic lesions show marked changes in the relative intensity of two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) signals when compared with those from healthy arterial tissue. The observed sharp increase of the relative SHG signal intensity in a plaque is in agreement with the known pathology of early lesions which have increased collagen content.

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Michael S. D. Smith, Alex C. T. Ko, Andrew Ridsdale, Bernie Schattka, Adrian Pegoraro, Mark D. Hewko, Masashi Shiomi, Albert Stolow, and Michael G. Sowa "A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions", Proc. SPIE 7386, Photonics North 2009, 73860I (4 August 2009); https://doi.org/10.1117/12.840500

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