The accuracy of a commercial spectrophotometer with single integrating sphere for measuring optical properties of turbid sample

Yang Zhang; Xiang Wen; ZhuDi Xu; Dan Zhu

doi:10.1117/12.841821

23 February 2010 The accuracy of a commercial spectrophotometer with single integrating sphere for measuring optical properties of turbid sample

Yang Zhang, Xiang Wen, ZhuDi Xu, Dan Zhu

Author Affiliations +

Proceedings Volume 7562, Optical Interactions with Tissues and Cells XXI; 756219 (2010) https://doi.org/10.1117/12.841821
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A commercial spectrophotometer with an integrating sphere is widely used to measure the spectra of transmittance and reflectance of turbid sample, and then the optical properties can be deduced by inverse adding-doubling algorithm. Unfortunately, the accuracy of the measurement is not been elucidated completely. What's more, for the system, there still exists some other limits, i.e., the incident light is not collimated and size of light spot is too large compared with the sample port. Thus, the purpose of our study is to evaluate the accuracy of the commercial spectrophotometer with an integrating sphere for measuring optical properties of tissue phantom. Two phantom materials, Intralipid and Evans blue, and the mixture of these two, were chosen for the experiments. Mie theory was also introduced to calculate the reduced scattering coefficient according to the particle size distribution. The results show that the phantom measurement in conjunction with IAD algorithm enable the determination of scattering coefficient μ_s' to be better than 5% accuracy, absorption coefficient μ_a to be better than 10% accuracy when the optical depth of sample is between 1 and 10, and the albedo is bigger than 0.4. For scattering of samples during 1-5 mm^-1, the error of μ_s' is smaller than 4%; whereas for absorbing of samples >0.4 mm^-1, the maximum error is smaller than 8.3%. Therefore, spectrophotometer with an integrating sphere technique combined IAD algorithm is applicable for the measurements of optical properties for most tissue, and its repeatability and accuracy is good in proper scope of the optical depth and albedo.

Citation Download Citation

Yang Zhang, Xiang Wen, ZhuDi Xu, and Dan Zhu "The accuracy of a commercial spectrophotometer with single integrating sphere for measuring optical properties of turbid sample", Proc. SPIE 7562, Optical Interactions with Tissues and Cells XXI, 756219 (23 February 2010); https://doi.org/10.1117/12.841821

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available