Cellular spectroscopy and multi-angle light scattering by flow cytometry: optical test bench as a developmental tool

Gordon Wiegand; Itzhak Avital

doi:10.1117/12.849163

25 February 2010 Cellular spectroscopy and multi-angle light scattering by flow cytometry: optical test bench as a developmental tool

Gordon Wiegand, Itzhak Avital

Author Affiliations +

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 75681J (2010) https://doi.org/10.1117/12.849163
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We designed and fabricated a flow cytometry work bench for the purpose of testing optical schemes utilized in two specialized flow cytometry techniques. Fluorescent spectroscopy and multi-angle scattering of laser light has potential for generating novel classes of cell population information. The acquisition of full fluorescence spectrum of dye bound to living cells may associate specific function to spectral shift. Similarly, detection of 2 discrete angles of light scatter was shown to describe internal structures of cells based on refractive and diffractive properties. We utilized a flow cytometry electro- cells based on refractive and diffractive properties. We utilized a flow cytometry electro- optical test bench to develop and describe alternate light collection schemes for these two techniques. Mouse bone marrow was chosen as the subject of analysis because it contains a consistent mix of cells that possess distinct biophysical properties. More importantly, these cells are the source of various biological systems that are targeted by environmental stress and clinical disease.

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Gordon Wiegand and Itzhak Avital "Cellular spectroscopy and multi-angle light scattering by flow cytometry: optical test bench as a developmental tool", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75681J (25 February 2010); https://doi.org/10.1117/12.849163

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KEYWORDS

Light scattering

Flow cytometry

Bone

Spectroscopy

Laser scattering

Luminescence

Diffraction

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