Autophagy induction upon reactive oxygen species in Cd-stressed Arabidopsis thaliana

WeiNa Zhang; WenLi Chen

doi:10.1117/12.841394

25 February 2010 Autophagy induction upon reactive oxygen species in Cd-stressed Arabidopsis thaliana

WeiNa Zhang, WenLi Chen

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 75681Y (2010) https://doi.org/10.1117/12.841394
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Autophagy is a protein degradation process in which cells recycle cytoplasmic contents when subjected to environmental stress conditions or during certain stages of development. Upon the induction of autophagy, a double membrane autophagosome forms around cytoplasmic components and delivers them to the vacuole for degradation. In plants, autophagy has been shown previously to be induced during abiotic stresses including oxidative stress. Cd, as a toxicity heavy metal, resulted in the production of reactive oxygen species (ROS). In this paper, we demonstrated that ROS contributed to the induction of autophagy in Cd-stressed Arabidopsis thaliana. However, pre-incubation with ascorbic acid (AsA, antioxidant molecule) and catalase (CAT, a H₂O₂-specific scavenger) decreased the ROS production and the number of autolysosomal-like structures. Together our results indicated that the oxidative condition was essential for autophagy, as treatment with AsA and CAT abolished the formation of autophagosomes, and ROS may function as signal molecules to induce autophagy in abiotic stress.

Citation Download Citation

WeiNa Zhang and WenLi Chen "Autophagy induction upon reactive oxygen species in Cd-stressed Arabidopsis thaliana", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75681Y (25 February 2010); https://doi.org/10.1117/12.841394

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