Inertial microfluidics for flow cytometry

Dino Di Carlo

doi:10.1117/12.861514

24 August 2010 Inertial microfluidics for flow cytometry

Dino Di Carlo

Proceedings Volume 7759, Biosensing III; 77590K (2010) https://doi.org/10.1117/12.861514
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

Inertial components of the Navier-Stokes equations are usually not considered in microfluidic flows but have recently been shown to be of great practical use for continuous manipulation of particles and cells. After introducing the physical basis of the counter-intuitive self focusing of particles in a single inlet flow, I will discuss our current best focusing systems, and I will present results on using inertial focusing to create an extreme throughput flow cytometer for blood analysis. This system is an imaging cytometer implementation that can image 1 million focused blood cells per second, with the capability to increase to 20 million cells per second with appropriate wide-field of view imaging systems. The microfluidic device consists of 256 parallel high-aspect ratio microchannels in each of which two streams of focused cells assemble. These cells also form regular trains in the direction of flow such that cell coincidence is a rare occurrence, far below Poisson statistics suggest. Controlled inertially focused streams of particles are poised to provide next-generation filter-less filters and simplified flow cytometry instruments which ultimately may aid in water treatment environmental cleanup and cost-effective medical diagnostics.

Citation Download Citation

Dino Di Carlo "Inertial microfluidics for flow cytometry", Proc. SPIE 7759, Biosensing III, 77590K (24 August 2010); https://doi.org/10.1117/12.861514

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