Imaging microscopic structures in pathological retinas using a flood-illumination adaptive optics retinal camera

Clément Viard; Kiyoko Nakashima; Barbara Lamory; Michel Pâques; Xavier Levecq; Nicolas Château

doi:10.1117/12.874766

11 February 2011 Imaging microscopic structures in pathological retinas using a flood-illumination adaptive optics retinal camera

Clément Viard, Kiyoko Nakashima, Barbara Lamory, Michel Pâques, Xavier Levecq, Nicolas Château

Author Affiliations +

Proceedings Volume 7885, Ophthalmic Technologies XXI; 788509 (2011) https://doi.org/10.1117/12.874766
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

This research is aimed at characterizing in vivo differences between healthy and pathological retinal tissues at the microscopic scale using a compact adaptive optics (AO) retinal camera. Tests were performed in 120 healthy eyes and 180 eyes suffering from 19 different pathological conditions, including age-related maculopathy (ARM), glaucoma and rare diseases such as inherited retinal dystrophies. Each patient was first examined using SD-OCT and infrared SLO. Retinal areas of 4°x4° were imaged using an AO flood-illumination retinal camera based on a large-stroke deformable mirror. Contrast was finally enhanced by registering and averaging rough images using classical algorithms. Cellular-resolution images could be obtained in most cases. In ARM, AO images revealed granular contents in drusen, which were invisible in SLO or OCT images, and allowed the observation of the cone mosaic between drusen. In glaucoma cases, visual field was correlated to changes in cone visibility. In inherited retinal dystrophies, AO helped to evaluate cone loss across the retina. Other microstructures, slightly larger in size than cones, were also visible in several retinas. AO provided potentially useful diagnostic and prognostic information in various diseases. In addition to cones, other microscopic structures revealed by AO images may also be of interest in monitoring retinal diseases.

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Clément Viard, Kiyoko Nakashima, Barbara Lamory, Michel Pâques, Xavier Levecq, and Nicolas Château "Imaging microscopic structures in pathological retinas using a flood-illumination adaptive optics retinal camera", Proc. SPIE 7885, Ophthalmic Technologies XXI, 788509 (11 February 2011); https://doi.org/10.1117/12.874766

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