Blind spectral unmixing to identify molecular signatures of absorbers in multispectral optoacoustic tomography

Stefan Morscher; Juergen Glatz; Nikolaos C. Deliolanis; Andreas Buehler; Daniel Razansky; Vasilis Ntziachristos

doi:10.1117/12.875290

28 February 2011 Blind spectral unmixing to identify molecular signatures of absorbers in multispectral optoacoustic tomography

Stefan Morscher, Juergen Glatz, Nikolaos C. Deliolanis, Andreas Buehler, Daniel Razansky, Vasilis Ntziachristos

Author Affiliations +

Proceedings Volume 7899, Photons Plus Ultrasound: Imaging and Sensing 2011; 78993D (2011) https://doi.org/10.1117/12.875290
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Multispectral optoacoustic (photoacoustic) tomography (MSOT) exploits the high resolutions provided by ultrasound imaging technology in combination with the more biologically relevant optical absorption contrast. Traces of molecules with different spectral absorption profiles, such as blood (oxy- and de-oxygenated) and biomarkers can be recovered using multiple wavelengths excitation and a set of methods described in this work. Three unmixing methods are examined for their performance in decomposing images into components in order to locate fluorescent contrast agents in deep tissue in mice. Following earlier works we find Independent Component Analysis (ICA), which relies on the strong criterion of statistical independence of components, as the most promising approach, being able to clearly identify concentrations that other approaches fail to see. The results are verified by cryosectioning and fluorescence imaging.

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Stefan Morscher, Juergen Glatz, Nikolaos C. Deliolanis, Andreas Buehler, Daniel Razansky, and Vasilis Ntziachristos "Blind spectral unmixing to identify molecular signatures of absorbers in multispectral optoacoustic tomography", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78993D (28 February 2011); https://doi.org/10.1117/12.875290

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