Adaptive optics confocal fluorescence microscopy with direct wavefront sensing for brain tissue imaging

Xiaodong Tao; Bautista Fernandez; Diana C. Chen; Oscar Azucena; Min Fu; Yi Zuo; Joel Kubby

doi:10.1117/12.876524

26 March 2011 Adaptive optics confocal fluorescence microscopy with direct wavefront sensing for brain tissue imaging

Xiaodong Tao, Bautista Fernandez, Diana C. Chen, Oscar Azucena, Min Fu, Yi Zuo, Joel Kubby

Proceedings Volume 7931, MEMS Adaptive Optics V; 79310L (2011) https://doi.org/10.1117/12.876524
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States

Abstract

Recently, there has been a growing interest in deep tissue imaging for the study of neurons. Unfortunately, because of the inhomogeneous refractive index of the tissue, the aberrations degrade the resolution and brightness of the final image. In this paper, we describe an adaptive optics confocal fluorescence microscope (AOCFM) which can correct aberrations based on direct wavefront measurements using a point source reference beacon and a Shack-Hartmann Wavefront Sensor (SHWS). Mouse brain tissues with different thicknesses are tested. After correction, both the signal intensity and contrast of the image are improved.

Citation Download Citation

Xiaodong Tao, Bautista Fernandez, Diana C. Chen, Oscar Azucena, Min Fu, Yi Zuo, and Joel Kubby "Adaptive optics confocal fluorescence microscopy with direct wavefront sensing for brain tissue imaging", Proc. SPIE 7931, MEMS Adaptive Optics V, 79310L (26 March 2011); https://doi.org/10.1117/12.876524

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