Characterization of atherosclerotic plaque-depositions by infrared, Raman and CARS microscopy

Christian Matthäus; Gero Bergner; Christoph Krafft; Benjamin Dietzek; Bernd F. M. Romeike; Bernhard R. Brehm; Jürgen Popp

doi:10.1117/12.890846

14 June 2011 Characterization of atherosclerotic plaque-depositions by infrared, Raman and CARS microscopy

Christian Matthäus, Gero Bergner, Christoph Krafft, Benjamin Dietzek, Bernd F. M. Romeike, Bernhard R. Brehm, Jürgen Popp

Author Affiliations +

Proceedings Volume 8087, Clinical and Biomedical Spectroscopy and Imaging II; 80871D (2011) https://doi.org/10.1117/12.890846
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Atherosclerotic plaques are mainly composed of proteoglycans, triglycerides, cholesterol, cholesterolester and crystalline calcium. From histopathological characterizations it is known that the composition of these atherosclerotic plaques can vary to a great extent, due to different risk factors as smoking, hyperlipedemia, or genetic background ect. The individual plaque components can be spectroscopically easily identified. Furthermore, spectroscopic imaging technologies offer the possibility to study the plaque compositions in a more quantitative manner than traditional staining techniques. Here, we compare the potential of IR, Raman and CARS microscopy to characterize the constitution of atherosclerotic plaques as well as the structure of the surrounding tissue. For data analysis and image reconstruction spectral decomposition algorithms such as vertex component analysis (VCA) were introduced. The results are in good agreement with the histopathology. Aim of the study is to correlate the compositional characteristics of atherosclerotic plaques with individual disease patterns.

Citation Download Citation

Christian Matthäus, Gero Bergner, Christoph Krafft, Benjamin Dietzek, Bernd F. M. Romeike, Bernhard R. Brehm, and Jürgen Popp "Characterization of atherosclerotic plaque-depositions by infrared, Raman and CARS microscopy", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871D (14 June 2011); https://doi.org/10.1117/12.890846

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