In vivo assessment of the structure of skin microcirculation by reflectance confocal-laser-scanning microscopy

Keiichi Sugata; Osamu Osanai; Hiromitsu Kawada

doi:10.1117/12.907570

9 February 2012 In vivo assessment of the structure of skin microcirculation by reflectance confocal-laser-scanning microscopy

Keiichi Sugata, Osamu Osanai, Hiromitsu Kawada

Author Affiliations +

Proceedings Volume 8207, Photonic Therapeutics and Diagnostics VIII; 82070U (2012) https://doi.org/10.1117/12.907570
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

One of the major roles of the skin microcirculation is to supply oxygen and nutrition to the surrounding tissue. Regardless of the close relationship between the microcirculation and the surrounding tissue, there are few non-invasive methods that can evaluate both the microcirculation and its surrounding tissue at the same site. We visualized microcapillary plexus structures in human skin using in vivo reflectance confocal-laser-scanning microscopy (CLSM), Vivascope 3000® (Lucid Inc., USA) and Image J software (National Institutes of Health, USA) for video image processing. CLSM is a non-invasive technique that can visualize the internal structure of the skin at the cellular level. In addition to internal morphological information such as the extracellular matrix, our method reveals capillary structures up to the depth of the subpapillary plexus at the same site without the need for additional optical systems. Video images at specific depths of the inner forearm skin were recorded. By creating frame-to-frame difference images from the video images using off-line video image processing, we obtained images that emphasize the brightness depending on changes of intensity coming from the movement of blood cells. Merging images from different depths of the skin elucidates the 3-dimensional fine line-structure of the microcirculation. Overall our results show the feasibility of a non-invasive, high-resolution imaging technique to characterize the skin microcirculation and the surrounding tissue.

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Keiichi Sugata, Osamu Osanai, and Hiromitsu Kawada "In vivo assessment of the structure of skin microcirculation by reflectance confocal-laser-scanning microscopy", Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82070U (9 February 2012); https://doi.org/10.1117/12.907570

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KEYWORDS

Skin

Capillaries

Tissues

Video

Image processing

Reflectivity

Visualization

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