Strip mosaicing confocal microscopy for rapid imaging over large areas of excised tissue

Sanjee Abeytunge; Yongbiao Li; Bjorg Larson; Gary Peterson; Ricardo Toledo-Crow; Milind Rajadhyaksha

doi:10.1117/12.909416

28 February 2012 Strip mosaicing confocal microscopy for rapid imaging over large areas of excised tissue

Sanjee Abeytunge, Yongbiao Li, Bjorg Larson, Gary Peterson, Ricardo Toledo-Crow, Milind Rajadhyaksha

Proceedings Volume 8214, Advanced Biomedical and Clinical Diagnostic Systems X; 821412 (2012) https://doi.org/10.1117/12.909416
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Confocal mosaicing microscopy is a developing technology platform for imaging tumor margins directly in fresh tissue, without the processing that is required for conventional pathology. Previously, basal cell carcinoma margins were detected by mosaicing of confocal images of 12 x 12 mm² of excised tissue from Mohs surgery. This mosaicing took 9 minutes. Recently we reported the initial feasibility of a faster approach called "strip mosaicing" on 10 x 10 mm² of tissue that was demonstrated in 3 minutes. In this paper we report further advances in instrumentation and software. Rapid mosaicing of confocal images on large areas of fresh tissue potentially offers a means to perform pathology at the bedside. Thus, strip mosaicing confocal microscopy may serve as an adjunct to pathology for imaging tumor margins to guide surgery.

Citation Download Citation

Sanjee Abeytunge, Yongbiao Li, Bjorg Larson, Gary Peterson, Ricardo Toledo-Crow, and Milind Rajadhyaksha "Strip mosaicing confocal microscopy for rapid imaging over large areas of excised tissue", Proc. SPIE 8214, Advanced Biomedical and Clinical Diagnostic Systems X, 821412 (28 February 2012); https://doi.org/10.1117/12.909416

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