Combined optical resolution photoacoustic and fluorescence micro-endoscopy

Peng Shao; Wei Shi; Parsin Hajireza; Roger J. Zemp

doi:10.1117/12.909777

23 February 2012 Combined optical resolution photoacoustic and fluorescence micro-endoscopy

Peng Shao, Wei Shi, Parsin Hajireza, Roger J. Zemp

Proceedings Volume 8223, Photons Plus Ultrasound: Imaging and Sensing 2012; 822318 (2012) https://doi.org/10.1117/12.909777
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We present a new micro-endoscopy system combining real-time C-scan optical-resolution photoacoustic micro-endoscopy (OR-PAME), and a high-resolution fluorescence micro-endoscopy system for visualizing fluorescently labeled cellular components and optically absorbing microvasculature simultaneously. With a diode-pumped 532-nm fiber laser, the OR-PAM sub-system is capable of imaging with a resolution of ~ 7μm. The fluorescence sub-system consists of a diode laser with 445 nm-centered emissions as the light source, an objective lens and a CCD camera. Proflavine, a FDA approved drug for human use, is used as the fluorescent contrast agent by topical application. The fluorescence system does not require any mechanical scanning. The scanning laser and the diode laser light source share the same light path within an optical fiber bundle containing 30,000 individual single mode fibers. The absorption of Proflavine at 532 nm is low, which mitigates absorption bleaching of the contrast agent by the photoacoustic excitation source. We demonstrate imaging in live murine models. The system is able to provide cellular morphology with cellular resolution co-registered with the structural and functional information given by OR-PAM. Therefore, the system has the potential to serve as a virtual biopsy technique, helping researchers and clinicians visualize angiogenesis, effects of anti-cancer drugs on both cells and the microcirculation, as well as aid in the study of other diseases.

Citation Download Citation

Peng Shao, Wei Shi, Parsin Hajireza, and Roger J. Zemp "Combined optical resolution photoacoustic and fluorescence micro-endoscopy", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 822318 (23 February 2012); https://doi.org/10.1117/12.909777

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